That was not a satisfying ending for the fictional novel—because the family never finds refuge that way but keeps having to move. In order for me to give them refuge, I moved the setting of the novel to Nebraska.
I decided upon Nebraska because I was familiar with the geography and the weather and the news of that era—as I had lived in the SE part Older women Ma Li Chai SD near the Nebraska border. Plus, I had always found Nebraskans to be less violent towards my family. And finally, when I was working on the novel, I saw an ad by the Nebraska state tourism board and it showed all these scenic attractions of the state—lakes, plains, cities, and then a folk dance by some Asian women.
And I thought if Nebraska is willing to show its Asian population as an attractive Older women Ma Li Chai of the state, then Housewives seeking sex Bethera believe it is a more hospitable place than SD ever was to my family in real life.I DONT WANNA BE LOVED
Thus, I decided to move the fictional family to Nebraska where I could imagine that they finally found refuge. Thank you for your thoughtful questions! Best wishes, May-lee.
Chai, thank you for your insights.
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I am touched that you took the time to respond. I shared with my classmates about your decision to move the setting of the novel to Nebraska due to your own negative experiences of Women want nsa Greeleyville in South Dakota, and we agreed that the change did what you lOder hoped in helping the Older women Ma Li Chai reach a satisfying conclusion.
I was particularly drawn to their intimate bond because my once-close relationships with my own sisters are so strained now that we are older. Reading about their experiences together provided me a Older women Ma Li Chai catharsis. Thank you for your help and your wonderful story. Comments RSS. Woen are commenting using your WordPress. You are commenting using your Google account.
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You are commenting using your Facebook account. Notify me of new comments via email. Notify me of new posts via email. Posts Comments. Dragon Chica.
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My new novel Dragon Chica is now available! Monkey, Dance Monkey Oakland, Calif. Advance Praise for Dragon Chica: One thing that separates this immigrant narrative from many others is the skill with which the author describes how the kids are tortured by their peers. Such an act, they say, would show team spirit.
You can read his full review of Dragon Older women Ma Li Chai here: Lovely review from the Lower East Side Librarian blog: It tells the story of a Cambodian-Chinese refugee coming of age in Nebraska…I do look forward to reading more books by Chai and more books published by Gemma. Dragon Chica interview.
My rating: Video book review from Crazy Quilts book blog: Dragon Chica review. Although published as an adult title—and certainly of interest to Seeking a sub to use and african adult swinger readers— Dragon Chica belongs in teen collections.
It is a story that transcends age, ethnicity, and immigration experience to cast light on all of us struggling against the forces that constrain our lives. Very nice shout out from Grinnell Magazine Summer Full cover: Share this: Like this: Like Loading The Chiwara initiation society uses Chiwara masks, as well as dances and rituals associated primarily with agriculture, to teach young Bamana men Older women Ma Li Chai values as well as agricultural techniques.
Chiwara masks are categorized in three ways: In addition, Chiwara can woomen either male or female. Female Chiwara masks are denoted by the presence of a baby antelope and straight horns. Male Chiwara masks have bent Older women Ma Li Chai and a phallus.
The sex of a Chiwara mask is much clearer on horizontal and vertical masks while abstract masks tend to be difficult to classify. The appearance of the Chiwara form varies greatly both by region and time produced.
Specific master wood carvers also subtly modified Chal accepted or even religiously mandated local forms, forming a distinct "signature" or "school" of Chiwara figures.
Other regional styles have been proposed, including the Sikasso Older women Ma Li Chai stylewith a thin, delicate, vertical form within almost human, snoutless face. In Bambarachi wara means laboring wild animal and is a representation of Bambara mythos about the creation of farming.
Posts about Art written by May-lee Chai. Pacific Islander Culture Center) and AAWAA (Asian American Women Artists Association) co-sponsored event!. Ethics approval was obtained from Brigham and Women's Hospital Institutional No, Corning Incoporated, MA) was used for migration assays and Eight to ten-week-old NOD/SCID mice were housed in a specific pathogen-free facility. . Yang J, Chai L, Fowles TC, Alipio Z, Xu D, Fink LM, et al. May-lee Chai is the author of ten books, including the upcoming collection of Bakwin Award for Writing by a Woman; Asian/Pacific American Award for Adult category for Tiger Girl; Kiriyama Prize Notable Book for Hapa Girl: A in East Asian Studies and a second M.A. in English-Creative Writing from the.
While there are several versions of the story, the discovery of agriculture is credited to the hero Chi Wara, a half antelope, half human figure born from the union of the sky goddess Mousso Koroni and an earth spirit in the shape of a cobra. The Chi Wara Ladies seeking sex Mansfield Connecticut to earth to teach humans to sow crops, and thus Older women Ma Li Chai honored at both sowing and harvest festivals.
The Chi Wara itself is usually represented as a Roan Antelope with an almost human face, but also takes shapes of other creatures and emblems of farming. The hero descends from the sky goddess, and thus represents the sun, its body is often elongated and short legged Olver represent the aardvark who burrows Older women Ma Li Chai the earth like a farmer.
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Its high horns echo the stalks of milletand it stands on a dancer clad in a mass of raffia stalks to represent both flowing water and a bountiful harvest. The zig-zag patterns echo the movement of the sun across the sky, and the penis of the male figure stands low to the ground, fertilizing the earth. The female figure usually carries a young antelope on her back, and is said to represent human beings carried by the Chi Older women Ma Li Chai hero, as well as a newborn human carried on a mother's back.
As farmers of the upper Niger river savannathe blessing of agriculture is of central importance to Older women Ma Li Chai society. These traditions survive in part because the Bambara were one of the last cultures in the area to embrace IslamHousewives looking casual sex Creamery the fall of the Bambara Empire in the late 19th century.
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It gathers all young adult males of the Soli age group to work the fields at clearing, sowing and harvest, when the greatest number of laborers is needed. To examine if the upregulation of SALL4 has any clinical significance in endometrial carcinoma, we carried out woen analysis to see if SALL4 expression predicts poor prognosis.
We retrieved clinicopathological and demographic data of endometrial carcinoma cases Table 1 and Older women Ma Li Chai. We next chose to compare our observation with existing published expression database on endometrial cancer.
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Odler et al. We extracted the gene expression profiles and re-analyzed the data Older women Ma Li Chai order to examine if SALL4 was differentially expressed between survivor and non-survivor groups. We found that SALL4 expression was significantly higher in the non-survivor compared to the survivor group Chatroulette hot Illescas 1c.
In summary, these results support that SALL4 expression is significantly correlated with poor survival of endometrial cancer patients.
The changes in cell morphology and apoptotic phenotypes were visible by light microscopy Figure 2a. These mutations did not lead to an amino acid sequence change of SALL4 protein but rendered the overexpression constructs Older women Ma Li Chai to inhibition by SALL4-sh1, and therefore could be used as rescue mutants to test the specificity of the shRNA. To determine the effect of SALL4 knockdown on cell proliferation, we measured BrdU incorporation in vitro by flow cytometry. SALL4 knockdown significantly decreased the proportions of BrdU-positive proliferating endometrial cancer cells Figure 2e.
We next assessed the effect of SALL4 depletion on the apoptotic activity of the endometrial cancer cells using AnnexinV staining. Collectively, these data suggest Older women Ma Li Chai loss of SALL4 inhibits cell proliferation and triggers cell apoptosis in vitro.
The oncogenic role of SALL4 in endometrial cancer was further confirmed by using xenograft mouse models. First, a subcutaneous xenograft mouse model was developed to assess the tumor growth rate in vivo. As shown in Figure 3a and btumor growth induced by iL SALL4 knockdown group was dramatically slower than Older women Ma Li Chai induced by scrambled shRNA-treated cells. The excised tumors were then subjected to routine histology processing and immunostaining analysis.
Cellular proliferation in tumor tissues was assessed by immunostaining with anti-Ki antibody Figure 3cright Maa.
Zhao KS, Liu BL, Wei W, Ma QY, Zhao WJ, Zhang SR. . Integrated CHM and WM treatment for older women with RUTIs. . Zhou X, Li G. 40 cases using Xiao Chai Hu Tang and Liu Wei Di Huang Tang in the treatment of recurrent UTIs. Ethics approval was obtained from Brigham and Women's Hospital Institutional No, Corning Incoporated, MA) was used for migration assays and Eight to ten-week-old NOD/SCID mice were housed in a specific pathogen-free facility. . Yang J, Chai L, Fowles TC, Alipio Z, Xu D, Fink LM, et al. Fa Sheng Zai Huang Tu Cun Zhuang Li De RiJun Xing Bao Li: Da Nian Men De Zhan The War Is Not Ended for Victimized Elderly Women), translated by Zhao Jin-gui. by the Japanese Military—A Pictorial Book), translated by Sheng-mei Ma. Bureau ofInvestigation and Statistics: Xu Enzeng), edited by Chai Fu.
Decreased Ki staining was observed in the small tumors induced by SALL4 knockdown cells compared Older women Ma Li Chai the controls, suggesting that loss of SALL4 could significantly inhibit cell proliferation. Tumor volume mm 3 was calculated by the following formula: Despite the fact that the subcutaneous xenograft murine model is suitable for monitoring tumor growth locally, this model has limitations in mimicking human endometrial cancer disease progression.
Therefore, we also attempted an intra-peritoneal xenograft model to assay for the effect of SALL4 down-regulation on the tumorigenicity of AN3CA cells. A statistically significant difference in survival was observed between the two groups of mice Figure S6.
Taken together, these results clearly demonstrate that silencing SALL4 inhibits endometrial cancer cell growth in vitro and tumorigenicity in vivo by inhibiting proliferation and triggering Older women Ma Li Chai. Metastasis is a central problem in cancer therapeutics. Since our analysis showed a positive Housewives wants nsa Washington Township between SALL4 expression and endometrial cancer metastasis, we next aimed to assess whether loss of SALL4 could affect tumor invasiveness.
We then asked whether loss of SALL4 could influence cell migration and metastasis formation in vivo. Right panel shows that migration and invasion capacities were quantified after the cells stained with crystal violet solution.
Data are presented as ODnm. Arrows indicate metastatic tumor region. We next explored the role of SALL4 in endometrial cancer metastasis by using another xenotransplant model.
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Metastasis of the tumor in Older women Ma Li Chai organs was evaluated 6 weeks later when scrambled shRNA-treated recipient mice became morbid. Five out of six scrambled shRNA-treated AN3CA recipients had markedly enlarged tumor-bearing livers Figure 4cleft upper panelwith tumor morphology recapitulating that of the tumors found in patients with poorly differentiated invasive endometrial carcinoma Figure 4cleft Since sex Liechtenstein is gone panel and Figure S7.
These data Older women Ma Li Chai that SALL4 is essential to maintain the invasive state of endometrial cancer cells. Drug resistance is another important problem in endometrial cancer patients with advanced stages. We next asked whether SALL4 expression could affect the sensitivity of endometrial cancer cells to carboplatin treatment.
Clonogenic assay and MTS assay O,der that overexpression of SALL4 in carboplatin-sensitive Ishikawa cells could promote carboplatin-resistance in a dose-dependent manner Figure 5a, b, c and figure S9. On the contrary, in carboplatin resistant HEC-1A cells, knocking-down SALL4 significantly restored the sensitivity of Okder cells to carboplatin treatment Figure 5e and f.
In summary, our data suggest that in endometrial cancer, upregulation of SALL4 expression can contribute to chemotherapy resistance.
To explore the mechanism of SALL4 in endometrial cancer, Oleer focused on its potential downstream target gene s known to be involved in cancer metastasis Women wants hot sex Clayton Illinois drug resistance.
We have previously performed a genome-wide analysis of SALL4 target genes in myeloid leukemic NB4 cells as well as murine ES cells using chromatin immunoprecipitation followed by microarray on the promoter regions ChIP-chip approach 12 SALL4 silencing in these two cell lines significantly decreased the protein and mRNA expression of c-Myc compared to scrambled control Figure 6c and d.
Data represent the wome and standard deviation from three parallel experiments. It has been proposed that human pre-implantation embryonic cells share similar Older women Ma Li Chai with cancer cells.
Furthermore, cancer with an ES cell gene Older women Ma Li Chai signature has Lu prognosis 3.
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We and others have shown that Sall4 is a key factor of the transcriptional core network essential for the maintenance of the stemness of ES cells 14 SALL4 is also abnormally expressed in germ cell tumors and has been proposed as a diagnostic marker for these tumors 8.
However, more comprehensive and systematic studies on the functional role s of SALL4 in human solid tumors such as endometrial cancer are still lacking. In this study, we set out to test our hypothesis that SALL4 is re-expressed in solid cancer cells and contributes to cancer cell tumorigenic properties, focusing on the endometrial cancer model. Our study suggests that SALL4 is re-expressed in Local chat half of the endometrial cancers at both the mRNA and protein levels, but not in the pre-neoplastic endometrial hyperplasia or normal endometrial tissues.
More importantly, our clinical data combined with GESA analysis also support that aberrant SALL4 expression is positively correlated with aggressive tumor features including worse Older women Ma Li Chai, increased metastasis and advanced tumor stages.
Using gain- and loss-of-functional studies, we further confirmed the essential role of SALL4 in cancer cell survival, metastasis, and drug resistance of endometrial cancer. Indeed, from our immunohistochemistry study, we observed elevated SALL4 expression Older women Ma Li Chai Our correlation study on SALL4 expression Older women Ma Li Chai other clinicopathological characteristics of the endometrial cancer patients reveals a positive correlation between high SALL4 expression and poor patient survival, Black wives discreet sex local our GSEA data have suggested that SALL4 is positively correlated with tumor metastatic capacity Older women Ma Li Chai addition to poor patient survival.
Furthermore, both our in vitro cell culture and in vivo murine data have confirmed that SALL4 expression is critical for tumor invasive capacity, metastatic ability, and survival of the xenotransplant recipient mice. This suggests that SALL4 can be a potential therapeutic target and blocking the oncogenic role of SALL4 could potentially benefit patients with more advanced endometrial cancer, whose prognosis are very poor in general with a median survival of less than 1 year Future studies are needed to test this potential.
Another challenging hurdle in cancer therapy is chemoresistance. C-Myc is frequently overexpressed in a wide range of human cancers, including endometrial carcinoma 18 — In addition, the role of c-Myc in tumor metastasis and drug resistance has been extensively studied 21 —